Journal
NATURE METHODS
Volume 14, Issue 4, Pages 388-+Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nmeth.4183
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Funding
- DARPA [W911NF-14-1-0012]
- NIH/NIBIB [R01EB014873]
- NIH/NINDS [U01NS090530]
- Intelligence Advanced Research Projects Activity (IARPA) via Department of Interior/Interior Business Center (DoI/IBC) [D16PC00003]
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High-resolution optical imaging is critical to understanding brain function. We demonstrate that three-photon microscopy at 1,300-nm excitation enables functional imaging of GCaMP6s-labeled neurons beyond the depth limit of two-photon microscopy. We record spontaneous activity from up to 150 neurons in the hippocampal stratum pyramidale at similar to 1-mm depth within an intact mouse brain. Our method creates opportunities for noninvasive recording of neuronal activity with high spatial and temporal resolution deep within scattering brain tissues.
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