Journal
NATURE METHODS
Volume 14, Issue 4, Pages 407-+Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nmeth.4184
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Funding
- Ontario Institute for Cancer Research through Government of Ontario
- Johns Hopkins University Catalyst award
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In nanopore sequencing devices, electrolytic current signals are sensitive to base modifications, such as 5-methylcytosine (5-mC). Here we quantified the strength of this effect for the Oxford Nanopore Technologies MinION sequencer. By using synthetically methylated DNA, we were able to train a hidden Markov model to distinguish 5-mC from unmethylated cytosine. We applied our method to sequence the methylome of human DNA, without requiring special steps for library preparation.
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