Journal
NATURE METHODS
Volume 14, Issue 4, Pages 443-+Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nmeth.4195
Keywords
-
Categories
Funding
- Office of Science, Office of Basic Energy Sciences (OBES), Division of Chemical Sciences, Geosciences and Biosciences (CSGB) of the US Department of Energy (DOE) [DE-AC02-05CH11231]
- US National Institutes of Health (NIH) [GM110501, GM102520, GM117126, GM055302]
- Ruth L. Kirschstein National Research Service Award [5 F32 GM116423-02]
- Human Frontiers Science Project [RGP0063/2013 310]
- Science and Technology Center program of the US National Science Foundation (NSF) through BioXFEL [1231306]
- NSF [MCB-1329956]
- Gulf Coast Consortia on the Houston Area Molecular Biophysics Program (NIHGMS) [T32GM008280]
- NIH NRSA grant [GM113389-01]
- Brookhaven National Laboratory (BNL)-US DOE, Laboratory Directed Research and Development [11-008]
- NIH-NCRR [2-P41-RR012408]
- NIH-National Institute of General Medical Sciences (NIGMS) [8P41GM103473-16, Y1GM008003]
- US DOE, Office of Biological and Environmental Research (OBER) grant [FWP BO-70]
- Diamond Light Source
- Wellcome Trust
- Biotechnology and Biological Sciences Research Council [102593]
- Knut and Alice Wallenberg Foundation
- Swedish Cancer Society
- Wenner-Gren foundations
- Swedish Research Council [2013-541, 2013-5884]
- US DOE, Office of Science, Division of CSGB
- DFG-Cluster of Excellence 'UniCat'
- Humboldt Universitat Berlin [Sfb1078, TP A5]
- Solar Fuels Strong Research Environment (Umea University)
- Artificial Leaf Project (K&A Wallenberg Foundation) [2011.0055]
- Energimyndigheten [36648-1]
- Office of Science, DOE [DE-AC02-05CH11231]
- DOE OBES
- DOE Office of Biological and Environmental Research
- NIH [P41GM103393]
- US DOE, Office of Science, OBES [DE-AC02-76SF00515]
- Life Science and Biomedical Technology Research (LSBR) program at the National Synchrotron Light Source II (NSLS-II), under a DOE BER contract [DE-SC0012704]
- DOE BES contract [DE-AC02-98CH10886]
- NIH-NIGMS [P41GM111244]
- Div Of Molecular and Cellular Bioscience
- Direct For Biological Sciences [1623935] Funding Source: National Science Foundation
Ask authors/readers for more resources
X-ray crystallography at X-ray free-electron laser sources is a powerful method for studying macromolecules at biologically relevant temperatures. Moreover, when combined with complementary techniques like X-ray emission spectroscopy, both global structures and chemical properties of metalloenzymes can be obtained concurrently, providing insights into the interplay between the protein structure and dynamics and the chemistry at an active site. The implementation of such a multimodal approach can be compromised by conflicting requirements to optimize each individual method. In particular, the method used for sample delivery greatly affects the data quality. We present here a robust way of delivering controlled sample amounts on demand using acoustic droplet ejection coupled with a conveyor belt drive that is optimized for crystallography and spectroscopy measurements of photochemical and chemical reactions over a wide range of time scales. Studies with photosystem IIII, the phytochrome photoreceptor, and ribonucleotide reductase R2 illustrate the power and versatility of this method.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available