Journal
NATURE METHODS
Volume 14, Issue 8, Pages 789-+Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.4342
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Funding
- Fundacio Cellex Barcelona
- European Union Seventh Framework Programme under the European Research Council [337191-MOTORS]
- Spanish Ministry of Economy and Competitiveness
- Fondo Europeo de Desarrollo Regional (FEDER) [FIS2015-63550-R]
- 'Severo Ochoa' Programme for Centres of Excellence in RD [SEV-2015-0522]
- European Social Fund (ESF) through the Ramon y Cajal program [RYC-2015-17896]
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Single-molecule-based super-resolution microscopy offers researchers a unique opportunity to quantify protein copy number with nanoscale resolution. However, while fluorescent proteins have been characterized for quantitative imaging using calibration standards, similar calibration tools for immunofluorescence with small organic fluorophores are lacking. Here we show that DNA origami, in combination with GFP antibodies, is a versatile platform for calibrating fluorophore and antibody labeling efficiency to quantify protein copy number in cellular contexts using super-resolution microscopy.
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