Journal
NATURE METHODS
Volume 14, Issue 4, Pages 391-+Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nmeth.4222
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Funding
- Natural Sciences and Engineering Research Council of Canada (NSERC) [RGPIN 288338-2010, 435762-2013]
- Canadian Institutes for Health Research (CIHR) [MOP-123514, 136812]
- Brain Canada Platform Support Grant
- Alberta Innovates Technology Futures (AITF)
- University of Alberta
- Alberta Ministry of Advanced Education
- Alberta Glycomics Centre
- Swiss National Science Foundation (SNF) [31003A 144048]
- European Union [334552]
- CIHR
- Alberta Innovates Health Solutions (AIHS)
- Swiss National Science Foundation (SNF) [31003A_144048] Funding Source: Swiss National Science Foundation (SNF)
- Alberta Innovates [201400500] Funding Source: researchfish
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To expand the range of experiments that are accessible with optogenetics, we developed a photocleavable protein (PhoCl) that spontaneously dissociates into two fragments after violet-light-induced cleavage of a specific bond in the protein backbone. We demonstrated that PhoCl can be used to engineer light-activatable Cre recombinase, Gal4 transcription factor, and a viral protease that in turn was used to activate opening of the large-pore ion channel Pannexin-1.
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