4.8 Article

CRISPR-Cas9-based photoactivatable transcription systems to induce neuronal differentiation

Journal

NATURE METHODS
Volume 14, Issue 10, Pages 963-+

Publisher

NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.4430

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Funding

  1. Japan Science and Technology Agency (START)
  2. Japan Science and Technology Agency (CREST) [JPMJCR1653]
  3. Japan Society for the Promotion of Science (JSPS) [15J05897, 15J08446]
  4. Grants-in-Aid for Scientific Research [15J05897, 15J08446] Funding Source: KAKEN

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Our improved CRISPR-Cas9-based photoactivatable transcription systems, CPTS2.0 and Split-CPTS2.0, enable high blue-light-inducible activation of endogenous target genes in various human cell lines. We achieved reversible activation of target genes with CPTS2.0 and induced neuronal differentiation in induced pluripotent stem cells (iPSCs) by upregulating NEUROD1 with Split-CPTS2.0.

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