Journal
NATURE METHODS
Volume 14, Issue 10, Pages 959-+Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.4396
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Funding
- Stanford Alzheimer's Disease Research Center [NIH P50 AG047366]
- Pacific Udall Center for Excellence in Parkinson's Disease Research [NIH P50 NS062684]
- Leukemia & Lymphoma Society Career Development Program
- US National Institutes of Health (NIH) training grant [R25CA180993]
- NIH [P50-HG007735, UM1HG00943, U19AI057266]
- National Institute on aging grant [RF1 AG053959]
- Rita Allen Foundation
- Human Frontier Science Program
- National Science Foundation Graduate Research Fellowship Program
- US Department of Defense National Defense Science and Engineering Graduate (NDSEG) Fellowship
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We present Omni-ATAC, an improved ATAC-seq protocol for chromatin accessibility profiling that works across multiple applications with substantial improvement of signal-to-background ratio and information content. The Omni-ATAC protocol generates chromatin accessibility profiles from archival frozen tissue samples and 50-mu m sections, revealing the activities of disease-associated DNA elements in distinct human brain structures. The Omni-ATAC protocol enables the interrogation of personal regulomes in tissue context and translational studies.
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