Journal
NATURE METHODS
Volume 15, Issue 2, Pages 115-+Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nmeth.4536
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Funding
- NIGMS [R01 GM124472]
- NSF [1350829, 1553344, IDBR 1353939, GRFP DGE-1444932, 1150235]
- NCI [1F99CA223074-01]
- Alfred P. Sloan Foundation
- NIH [RO1HL121264, RO1 HL130918, U01-HL117721, U54HL112309]
- Direct For Biological Sciences
- Div Of Biological Infrastructure [1353939] Funding Source: National Science Foundation
- Direct For Biological Sciences
- Div Of Molecular and Cellular Bioscience [1553344] Funding Source: National Science Foundation
- Div Of Chem, Bioeng, Env, & Transp Sys
- Directorate For Engineering [1150235] Funding Source: National Science Foundation
- Div Of Molecular and Cellular Bioscience
- Direct For Biological Sciences [1350829] Funding Source: National Science Foundation
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Mechanical forces are integral to many biological processes; however, current techniques cannot map the magnitude and direction of piconewton molecular forces. Here, we describe molecular force microscopy, leveraging molecular tension probes and fluorescence polarization microscopy to measure the magnitude and 3D orientation of cellular forces. We mapped the orientation of integrin-based traction forces in mouse fibroblasts and human platelets, revealing alignment between the organization of force-bearing structures and their force orientations.
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