Journal
NATURE METHODS
Volume 15, Issue 1, Pages 47-+Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.4509
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Funding
- CONACYT-Mexico
- PEW Latin American Fellows Program
- NSF GRFP fellowship
- Harvard University Ashford Fellowship
- NSF [1615487, 1410176]
- DARPA [HR0011-16-2-0049]
- NIH [1R21AI094363]
- NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES [R21AI094363] Funding Source: NIH RePORTER
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The slow maturation time of fluorescent proteins (FPs) limits the temporal accuracy of measurements of rapid processes such as gene expression dynamics and effectively reduces fluorescence signal in growing cells. We used high-precision time-lapse microscopy to characterize the maturation kinetics of 50 FPs that span the visible spectrum at two different temperatures in Escherichia coli cells. We identified fast-maturing FPs from this set that yielded the highest signal-to-noise ratio and temporal resolution in individual growing cells.
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