Journal
NATURE METHODS
Volume 14, Issue 6, Pages 593-+Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH.4261
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Funding
- HHMI-Simons Faculty Scholars Program
- NIH Director's Pioneer Award [1DP1NS087724]
- New York Stem Cell Foundation Robertson Award
- US Army Research Laboratory
- US Army Research Office [W911NF1510548]
- US-Israel Binational Science Foundation [2014509]
- Picower Institute Innovation Fund
- IARPA [D16PC00008]
- NIH [1R01MH110932, 1R43MH101943, 1R01MH103910, 1R01EY023173, 2R01DA029639, R21GM114852, RO1MH110932]
- JET A.F. Harvey Prize
- Open Philanthropy Project
- Halis Family Foundation
- MIT Media Lab
- Simons Postdoctoral Fellowship
- NSF Fellowship
- Poitras Fellowship
- Samsung Scholarships
- Hertz Foundation fellowships
- Center for Neuroscience Imaging Research
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We recently developed a method called expansion microscopy, in which preserved biological specimens are physically magnified by embedding them in a densely crosslinked polyelectrolyte gel, anchoring key labels or biomolecules to the gel, mechanically homogenizing the specimen, and then swelling the gel-specimen composite by-4.5x in linear dimension. Here we describe iterative expansion microscopy (iExM), in which a sample is expanded-20x. After preliminary expansion a second swellable polymer mesh is formed in the space newly opened up by the first expansion, and the sample is expanded again. iExM expands biological specimens similar to 4.5 x 4.5, or similar to 20x, and enables similar to 25-nm-resolution imaging of cells and tissues on conventional microscopes. We used iExM to visualize synaptic proteins, as well as the detailed architecture of dendritic spines, in mouse brain circuitry.
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