Journal
NATURE CHEMICAL BIOLOGY
Volume 13, Issue 8, Pages 839-+Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nchembio.2410
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Funding
- NIH [R37 AI091476, P01 AI100263]
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Cpf1 is a CRISPR effector protein that has greater specificity than Streptococcus pyogenes Cas9 (SpCas9) in genome-editing applications. Here we show that Lachnospiraceae bacterium (Lb) and Acidaminococus sp. (As) Cpf1 orthologs have RNase activities that can excise multiple CRISPR RNAs (crRNAs) from a single RNA polymerase II-driven RNA transcript expressed in mammalian cells. This property simplifies modification of multiple genomic targets and can be used to increase the efficiency of Cpf1-mediated editing.
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