Journal
NATURE CHEMICAL BIOLOGY
Volume 14, Issue 1, Pages 79-+Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NCHEMBIO.2509
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Funding
- Canadian Institutes of Health Research (CIHR) [TGC 114045, MOP 43998]
- Canadian Glycomics Network
- University of Toronto
- Ontario Graduate Scholarship Program
- CIHR
- Canada Research Chair
- United States Department of Energy Office of Biological and Environmental Research
- National Institutes of Health National Centre for Research Resources
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O-Acetylation of the secondary cell wall polysaccharides (SCWP) of the Bacillus cereus group of pathogens, which includes Bacillus anthracis, is essential for the proper attachment of surface-layer (S-layer) proteins to their cell walls. Using a variety of pseudosubstrates and a chemically synthesized analog of SCWP, we report here the identification of PatB1 as a SCWP O-acetyltransferase in Bacillus cereus. Additionally, we report the crystal structure of PatB1, which provides detailed insights into the mechanism of this enzyme and defines a novel subfamily of the SGNH family of esterases and lipases. We propose a model for the O-acetylation of SCWP requiring the translocation of acetyl groups from a cytoplasmic source across the plasma membrane by PatA1 and PatA2 for their transfer to SCWP by PatB1.
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