Journal
NATURE BIOTECHNOLOGY
Volume 35, Issue 5, Pages 441-+Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nbt.3833
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Funding
- Cabinet Office, Government of Japan
- Bio-oriented Technology Research Advancement Institution, NARO
- Ministry of Education, Culture, Sports and Technology (MEXT) of Japan
- Platform Project for Supporting in Drug Discovery and Life Science Research (Platform for Drug Discovery, Informatics, and Structural Life Science) from the Japan Agency for Medical Research and Development (AMED)
- JSPS KAKENHI [26119710, 16K14654]
- New Energy and Industrial Technology Development Organization (NEDO)
- Grants-in-Aid for Scientific Research [16K07560, 16K14654, 15K18647, 26119710] Funding Source: KAKEN
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We applied a fusion of CRISPR-Cas9 and activation-induced cytidine deaminase (Target-AID) for point mutagenesis at genomic regions specified by single guide RNAs (sgRNAs) in two crop plants. In rice, we induced multiple herbicide-resistance point mutations by multiplexed editing using herbicide selection, while in tomato we generated marker-free plants with homozygous heritable DNA substitutions, demonstrating the feasibility of base editing for crop improvement.
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