Journal
NATURE BIOTECHNOLOGY
Volume 35, Issue 6, Pages 523-529Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nbt.3873
Keywords
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Categories
Funding
- Defense for Research & Engineering under Air Force [FA8721-05-C-0002]
- National Cancer Institute of the National Institutes of Health [R01CA173712]
- National Institute of General Medical Sciences of the National Institutes of Health [P50GM098792]
- National Science Foundation [1124247, 1522074, 1521925, 1521759]
- Direct For Computer & Info Scie & Enginr
- Division of Computing and Communication Foundations [1521759] Funding Source: National Science Foundation
- Direct For Computer & Info Scie & Enginr
- Division of Computing and Communication Foundations [1521925, 1522074, 1124247] Funding Source: National Science Foundation
- Division Of Computer and Network Systems
- Direct For Computer & Info Scie & Enginr [1446474] Funding Source: National Science Foundation
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Microfluidic devices have the potential to automate and miniaturize biological experiments, but open-source sharing of device designs has lagged behind sharing of other resources such as software. Synthetic biologists have used microfluidics for DNA assembly, cell-free expression, and cell culture, but a combination of expense, device complexity, and reliance on custom set-ups hampers their widespread adoption. We present Metafluidics, an open-source, community-driven repository that hosts digital design files, assembly specifications, and open-source software to enable users to build, configure, and operate a microfluidic device. We use Metafluidics to share designs and fabrication instructions for both a microfluidic ring-mixer device and a 32-channel tabletop microfluidic controller. This device and controller are applied to build genetic circuits using standard DNA assembly methods including ligation, Gateway, Gibson, and Golden Gate. Metafluidics is intended to enable a broad community of engineers, DIY enthusiasts, and other nontraditional participants with limited fabrication skills to contribute to microfluidic research.
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