Journal
NATURE BIOTECHNOLOGY
Volume 35, Issue 5, Pages 438-+Publisher
NATURE PORTFOLIO
DOI: 10.1038/nbt.3811
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Funding
- National Key Research and Development Program of China [2016YFD0101804]
- National Transgenic Science and Technology Program [2016ZX08010-002]
- Chinese Academy of Sciences [KFZD-SW-107, GJHZ1602]
- National Natural Science Foundation of China [31570369]
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Targeted base editing in plants without the need for a foreign DNA donor or double-stranded DNA cleavage would accelerate genome modification and breeding in a wide array of crops. We used a CRISPR-Cas9 nickase-cytidine deaminase fusion to achieve targeted conversion of cytosine to thymine from position 3 to 9 within the protospacer in both protoplasts and regenerated rice, wheat and maize plants at frequencies of up to 43.48%.
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