Journal
NATURE
Volume 548, Issue 7666, Pages 224-+Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nature23286
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Funding
- P-CREATE, Japan Agency for Medical Research and Development (AMED)
- SICORP, Japan Agency for Medical Research and Development (AMED)
- JSPS KAKENHI [15H04721, 15H01352, 15J05792]
- Princess Takamatsu Cancer Research Fund
- Takeda Science Foundation
- Naito Foundation
- Core Center for iPS Cell Research
- Research Center Network for Realization of Regenerative Medicine, AMED
- AMED-CREST
- iPS Cell Research Fund
- Grants-in-Aid for Scientific Research [16H02593, 15J05792, 15H01352, 15H04721] Funding Source: KAKEN
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Inhibitors of Mek1/2 and Gsk3 beta, known as 2i, enhance the derivation of embryonic stem (ES) cells and promote ground-state pluripotency in rodents(1,2). Here we show that the derivation of female mouse ES cells in the presence of 2i and leukaemia inhibitory factor (2i/L ES cells) results in a widespread loss of DNA methylation, including a massive erasure of genomic imprints. Despite this global loss of DNA methylation, early-passage 2i/L ES cells efficiently differentiate into somatic cells, and this process requires genome-wide de novo DNA methylation. However, the majority of imprinting control regions (ICRs) remain unmethylated in 2i/L-ES-cell-derived differentiated cells. Consistently, 2i/L ES cells exhibit impaired autonomous embryonic and placental development by tetraploid embryo complementation or nuclear transplantation. We identified the derivation conditions of female ES cells that display 2i/L-ES-cell-like transcriptional signatures while preserving gamete-derived DNA methylation and autonomous developmental potential. Upon prolonged culture, however, female ES cells exhibited ICR demethylation regardless of culture conditions. Our results provide insights into the derivation of female ES cells reminiscent of the inner cell mass of preimplantation embryos.
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