Journal
NATURAL PRODUCT RESEARCH
Volume 32, Issue 24, Pages 2968-2972Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/14786419.2017.1395431
Keywords
Pestalotiopsis sp; cytotoxicity; demethylincisterol A(3); cell-cycle arrest; apoptosis
Categories
Funding
- National Natural Science Foundation of China [81660584]
- Young Talents' Science and Technology Innovation Project of Hainan Association for Science and Technology [201509]
- Key Research Program of Hainan Province [ZDYF2017099]
- Innovative Research Team grant of the Natural Science Foundation of Hainan University [hdkytg201705]
- Key Laboratory of Advanced Materials of Tropical Island Resources of Ministry of Education Open Fund in Hainan University [AM2017-01]
Ask authors/readers for more resources
Chemical examination of Chinese mangrove Rhizophora mucronata endophytic Pestalotiopsis sp., yielded 11 known metabolites with various structure types, including demethylincisterol A(3) (1), dankasterone B (2), (22E, 24R)-ergosta-7,9(11), 22-triene-3 beta, 5 alpha, 6 alpha-triol (3), ergosta-5,7,22-trien-3-ol (4), 5, 8-epidioxy-5, 8-ergosta-6, 22E-dien-3-ol (5), stigmastan-3-one (6), stigmast-4-en-3-one (7), stigmast-4-en-6 -ol-3-one (8), flufuran (9), (2-cis, 4-trans)-abscisic acid (10), similanpyrone B (11). Their structures were unambiguously elucidated on the basis of extensive NMR spectroscopic and mass spectrometric analyses. Compounds 1, 4, 6-9 showed significant in vitro cytotoxicity against the human cancer cell lines Hela, A549 and HepG, of which compound 1 was the most potential with IC50 values reaching nM degree ranging from 0.17 to 14.16 nM. Flow cytometric investigation demonstrated that compound 1 mainly inhibited cell cycle at G(0)/G(1) phase in a dose-dependent manner with a significant induction of apoptosis on the three tested cell lines. The involvement of the mitochondria in compound 1 induced apoptosis was investigated using MMP. We suggested that R. mucronata endophytic Pestalotiopsis sp. contained a potential anticancer compound demethylincisterol A(3). [GRAPHICS] .
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available