New chemical constituents from the fruits of Zanthoxylum armatum and its in vitro anti-inflammatory profile

Chemical investigations on the fruits of Zanthoxylum armatum Roxb. (Rutaceae) led to the isolation of two new constituents characterised as 2 alpha-methyl-2 beta-ethylene-3 beta-isopropyl-cyclohexan-1 beta, 3 alpha-diol (1) and phenol-O-beta-D-arabinopyranosyl-4 '-(3 '', 7 '', 11 '', 15 ''-tetramethyl)-hexadecan-1 ''-oate (2) along with known compounds m-methoxy palmityloxy benzene (3), acetyl phenyl acetate (4), linoleiyl-O-alpha-D-xylopyranoside (5), m-hydroxyphenoxy benzene (6) and palmitic acid (7). The chemical structures were established with the help of physical, chemical and spectroscopic methods. The anti-inflammatory potential of isolated compounds 1 and 2 was evaluated using in vitro target-based anti-inflammatory activity in LPS-stimulated primary peritoneal macrophages isolated from mice. Production of pro-inflammatory cytokines (TNF-alpha and IL-6) was significantly inhibited by the treatment of isolated compounds 1 and 2 in a dose-dependent manner. [GRAPHICS] .