Identification omega 3 and 6 on the positions sn-2 triacylglycerol Nile tilapia fish oil by hydrolysis using lipase from Mucor miehei

Omega 3 and 6 are unsaturated fatty acids needed by the body for cell formation and control of inflammation. In the molecules of omega 3 and 6 fats found in the position (stereospecific numbering) sn-1, 2 and 3. The position of fatty acids affects the digestion and absorption processes in the body. In the metabolism of fat in the body, fatty acids in the sn-2 position are not hydrolyzed so they can be absorbed properly. Therefore, it is necessary to identify the position of omega 3 and 6 fatty acids in fat molecules so that the potential of fish oils Nile tilapia as a source of omega 3 and 6 can be known. Fish oils Nile is extracted with the soxhletation method. The fatty acid composition was analyzed by gas chromatography (GC) which was previously esterified using BF3, for hydrolysis of fish oil using lipase enzyme. The results showed that the composition of unsaturated fatty acids was more than saturated fatty acids. Omega-3 and 6 fatty acids in the fat molecule are found more in the sn-2 than in the sn-1+sn-3 position. Seen from the composition and position fatty acids of Nile tilapia oil which contain omega 3 and 6 and there is a sn-2 position, so that more is absorbed in fat metabolism in the body. Thus fish oils Nile tilapia has the potential as a source of omega 3 and 6 from one of the freshwater fish that is widely consumed by the public.