Journal
NANOSCALE
Volume 9, Issue 1, Pages 183-192Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c6nr08145b
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Funding
- National Science Foundation [ACI-1053575]
- NSF at the Pittsburgh Supercomputing Center (PSC) [ACI-1445606]
- NIH/NIST Research Associateship Program through the National Academies of Sciences
- Intramural Research Program of the Eunice Kennedy Shriver National Institute of Child Health and Human Development, NIH
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We probe the energy landscape governing the passage of alpha-synuclein, a natural diblock copolymer-like polypeptide, through a nanoscale pore. alpha-Synuclein is an intrinsically disordered neuronal protein associated with Parkinson's pathology. The motion of this electrically heterogeneous polymer in the beta-barrel voltage-dependent anion channel (VDAC) of the mitochondrial outer membrane strongly depends on the properties of both the charged and uncharged regions of the alpha-synuclein polymer. We model this motion in two ways. First, a simple Markov model accounts for the transitions of the channel between the states of different occupancy by alpha-synuclein. Second, the detailed energy landscape of this motion can be accounted for using a drift-diffusion framework that incorporates the alpha-synuclein binding energy and the free energy cost of its confinement in the VDAC pore. The models directly predict the probability of alpha-synuclein translocation across the mitochondrial outer membrane, with immediate implications for the physiological role of alpha-synuclein in regulation of mitochondrial bioenergetics. Time-resolved measurements of the electrical properties of VDAC occupied by alpha-synuclein reveal distinct effects of the motion of the junction separating the differently charged regions of the polymer.
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