Journal
NANOSCALE
Volume 9, Issue 4, Pages 1607-1615Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c6nr09260h
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Funding
- National Basic Research Program of China [2012CB910601, 2013CB911201]
- National Nature Science Foundation [21190043, 21175131]
- National High Technology Research and Development Program of China [2012AA020202]
- Creative Research Group Project by NSFC [21321064]
- Innovation Method Fund of China [2012IM030900]
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Although selective enrichment of glycopeptides from complex biological samples is indispensable for mass spectrometry (MS)-based glycoproteomics, it still remains a great challenge due to the low abundance of glycoproteins and suppression of non-glycopeptides. In this study, silver nanoparticle-functionalized magnetic graphene oxide nanocomposites (GO/Fe3O4/PEI/Ag) were synthesized. Silver nanoparticles were generated in situ on the surface of magnetic graphene oxide using polyethylenimine as a reducing and stabilizing agent. The resulting material was used as an adsorbent for selective enrichment of glycopeptides. GO/Fe3O4/PEI/Ag nanocomposites offered excellent enrichment ability, which was attributed to the synergistic effect of polyethylenimine and silver nanoparticles. The nanocomposites showed superior specificity for glycopeptides even when non-glycopeptides were 100 times more concentrated than glycopeptides. The nanocomposites displayed advantages including rapid adsorption (1 min), low detection limit (25 fmol), repeatability (6 times), and high recovery (77.8%). Using these nanocomposites, 91 different glycoproteins and 136 N-linked glycopeptides were identified from among 20 mu g tryptic human serum proteins and this demonstrated the superior performance of the nanocomposites for glycopeptides enrichment.
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