Journal
NANOSCALE
Volume 9, Issue 41, Pages 15998-16004Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c7nr05545e
Keywords
-
Categories
Funding
- Korea Institute of Energy Technology Evaluation and Planning (KETEP)
- Ministry of Trade, Industry & Energy (MOTIE) of the Republic of Korea [20164030201060]
- Korea National Research Foundation (KNRF)
- Ministry of Education (MOE) of the Republic of Korea [2017R1D1A1B03032033, 2017R1D1A1B03030732]
- National Research Foundation of Korea [2017R1D1A1B03030732, 2017R1D1A1B03032033] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Ask authors/readers for more resources
Glucose oxidase (GOx) and polyacrylic acid (PAA) based water swellable non-toxic enzyme-polymer conjugate (PAA-GOx) was immobilized on a substrate consisting of graphene oxide (GO) and polyethyleneimine (PEI) (GO-PEI) and the electrochemical performances of the new catalyst were investigated. According to the measurements, although the amount of GOx immobilized on PAA-GOx was lower than that on glutaraldehyde (GA)-GOx, which is a conventionally used conjugate, its catalytic activity was 9.6 times higher than that of GA-GOx. The superior catalytic activity (102.0 mu A cm(-2), 20 mM of glucose) and glucose sensitivity (6.9 mu A cm(-2) mM(-1)) were due to its high swellability in water. Due to this, the PAA-GOx absorbs a large amount of aqueous glucose molecules and rapidly transfers them to the active site of GOx. Desirable hydrogen peroxide and glucose oxidation reactions are accordingly promoted. In addition, since PAA has abundant free carboxylic acid groups, the PAA-GOx forms covalent bonds with the GO-PEI to curtail the leaching-out of GOx molecules.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available