Journal
NANOSCALE
Volume 9, Issue 37, Pages 14094-14102Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c7nr03615a
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Funding
- National Research Foundation of Korea (NRF) - Korean Government [NRF-2016M3A9C6917402]
- Global Innovative Research Center (GiRC) through National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning [NRF-2012K1A1A2A01056093]
- National Research Foundation of Korea [2012K1A1A2A01056093, 2016M3A9C6917402] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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Nucleic acid-based technologies have been applied to numerous biomedical applications. As a novel material for target detection, DNA has been used to construct a barcode system with a range of structures. This paper reports multi-functionalized DNA nanospheres (DNANSs) by rolling circle amplification (RCA) with several functionalized nucleotides. DNANSs with a barcode system were designed to exhibit fluorescence for coding enhanced signals and contain biotin for more functionalities, including targeting through the biotin-streptavidin (biotin-STA) interaction. Functionalized deoxynucleotide triphosphates (dNTPs) were mixed in the RCA process and functional moieties can be expressed on the DNANSs. The anti-epidermal growth factor receptor antibodies (anti-EGFR Abs) can be conjugated on DNANSs for targeting cancer cells specifically. As a proof of concept, the potential of the multi-functional DNANS barcode was demonstrated by direct cell detection as a simple detection method. The DNANS barcode provides a new route for the simple and rapid selective recognition of cancer cells.
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