Journal
NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
Volume 13, Issue 5, Pages 1745-1759Publisher
ELSEVIER
DOI: 10.1016/j.nano.2017.02.016
Keywords
Non-mulberry silk fibroin; HAp; Nanoparticles; Bone tissue engineering
Funding
- Department of Biotechnology [BT/PR10941/MED/32/333/2014]
- DBT BioCARE [BT/Bio-CARe/01/817/2011-12]
- Indian Council of Medical Research, Govt. of India [5/13/12/2010/NCD-III]
- Department of Biotechnology
- Indian Council of Medical Research, Government of India
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Replacement and repair of ectopic bone defects and traumatized bone tissues are done using porous scaffolds and composites. The prerequisites for such scaffolds include high mechanical strength, osseoconductivity and cytocompatibility. The present work is designed to address such requirements by fabricating a reinforced cytocompatible scaffold. Biocompatible silk protein fibroin collected from tropical non-mulberry tasar silkworm (Antheraea mylitta) is used to fabricate fibroin-hydroxyapatite (HAp) nanocomposite particles using chemical precipitation method. In situ reinforcement of fibroin-HAp nanocomposite and external deposition of HAp particles on fibroin scaffold is carried out for comparative evaluations of bio-physical and biochemical characteristics. HAp deposited fibroin scaffolds provide greater mechanical strength and cytocompatibility, when compared with fibroin-HAp nanoparticles reinforced fibroin scaffolds. Minimal immune responses of both types of composite scaffolds are observed using osteoblast-macrophage co-culture model. Nanocomposite reinforced fibroin scaffold can be tailored further to accommodate different requirements depending on bone type or bone regeneration period. (c) 2017 Elsevier Inc. All rights reserved.
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