Journal
NANOMEDICINE
Volume 12, Issue 6, Pages 581-596Publisher
FUTURE MEDICINE LTD
DOI: 10.2217/nnm-2016-0344
Keywords
cell tracking; circulating tumor cells; microfluidics; nanoparticles; theranostics
Funding
- ComplexiTE [ERC-2012-ADG 20120216-321266]
- Portuguese Foundation for Science and Technology (FCT) [IF/00423/2012]
- LA ICVS/3Bs project [UID/Multi/50026/2013]
- Fundação para a Ciência e a Tecnologia [PTDC/SAU-GMG/113795/2009, UID/Multi/50026/2013] Funding Source: FCT
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Aim: Develop a platform composed of labeled dendrimer nanoparticles (NPs) and a microfluidic device for real-time monitoring of cancer cells fate. Materials & methods: Carboxymethylchitosan/ poly(amidoamine) dendrimer NPs were labeled with fluorescein-5(6)-isothiocyanate and characterized using different physicochemical techniques. After, HeLa, HCT-116 and U87MG were cultured in the presence of NPs, and cell viability and internalization efficiency in static (standard culture) and dynamic (microfluidic culture) conditions were investigated. Results: Cancer cells cultured with NPs in dynamic conditions were viable and presented higher internalization levels as compared with static 2D cultures. Conclusion: This work demonstrated that the proposed microfluidic-based platform allows real-time monitoring, which upon more studies, namely, the assessment of an anticancer drug release effect could be used for cancer theranostics.
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