Journal
5TH INTERNATIONAL CONFERENCE ON ENERGY AND ENVIRONMENT RESEARCH (ICEER 2018)
Volume 153, Issue -, Pages 248-252Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.egypro.2018.10.015
Keywords
Biodegradation; Bacillus subtilis C19; Kinetic
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Funding
- Universitas Indonesia, Ministry of Research, Technology and Higher Education Republic of Indonesia
- United States Agency for International Development (USAID) through the Sustainable Higher Education Research Alliance (SHERA) Program for Universitas Indonesia's Scientific Modeling, Application, Research and Training for City-centered Innovation and Techn [AID-497-A-1600004, IIE-00000078-UI-1]
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Biodegradation of pyrene compound was catalyzed by multi-component enzymes, and the initial dioxygenase gene was used as key Bacillus subtilis C19 enzyme for aromatic ring structure degradation. The bacterial cell growth rates were also analyzed; it was fitted using Monod, Mason-Mallis, Haldane, Andrews, and Aiba kinetics models. The un-competitive inhibition mechanism of high substrate concentration proposed by Andrews model could be best fitted to experimental bacterial growth kinetic data. Further, the maximum specific growth rate (mu(max)) has been found to be 0.0048 h(-1), the half velocity constant (K-s) was 0.0079 gL(-1), and the inhibition growth rate coefficient (K-i) was 0.2619 gL(-1). (C) 2018 The Authors. Published by Elsevier Ltd.
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