Journal
MOLECULES
Volume 22, Issue 1, Pages -Publisher
MDPI
DOI: 10.3390/molecules22010087
Keywords
genistein; melanoma; methoxyisoflavone; methyltransferase; tyrosinase
Funding
- National Scientific Council of Taiwan [MOST 105-2221-E-024-018-]
Ask authors/readers for more resources
Biotransformation of the soy isoflavone genistein by sequential 3-hydroxylation using recombinant Escherichia coli expressing tyrosinase from Bacillus megaterium and then methylation using another recombinant E. coli expressing O-methyltransferase from Streptomyces peucetius was conducted. The results showed that two metabolites were produced from the biotransformation, identified as 5,7,4-trihydroxy-3-methoxyisoflavone and 5,7,3-trihydroxy-4-methoxyisoflavone, respectively, based on their mass and nuclear magnetic resonance spectral data. 5,7,4-Trihydroxy-3-methoxyisoflavone showed potent antiproliferative activity toward mouse B16 melanoma cells with an IC50 value of 68.8 M. In contrast, the compound did not show any cytotoxicity toward mouse normal fibroblast cells, even at 350 M concentration. The results of the present study offer insight on the production of both 5,7,4-trihydroxy-3-methoxyisoflavone and 5,7,3-trihydroxy-4-methoxyisoflavone by two recombinant E. coli strains and the potential anti-melanoma applications of 5,7,4-trihydroxy-3-methoxyisoflavone.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available