Soluble Epoxide Hydrolase Inhibitory Activity of Components Isolated from Apios americana Medik

A new compound 1, 5-methoxy-2,5,7,4'-tetrahydroxy-coumaronochromone, along with seven known compounds (2-8), were isolated from Apios americana using open column chromatography. Their structures were established based on an analysis of 1D and 2D NMR, and MS spectra. Among these, two compounds 1 and 2 showed inhibitory activity on soluble epoxide hydrolase (sEH) at a concentration below 50 !AM. The respective competitive (1) and mixed (2) inhibitors were revealed to have Ki values of 21.0 +/- 0.8 and 14.5 +/- 1.5 mu M, based on the Dixon plot. The potential inhibitor (2) was visually presented in a predicted binding pose in the receptor by molecular docking. Additionally, molecular dynamics were performed for a detailed understanding of their complex by Gromacs 4.6.5 package.