Journal
MOLECULES
Volume 22, Issue 7, Pages -Publisher
MDPI
DOI: 10.3390/molecules22071084
Keywords
porphyrin; manganese; iron; DNA; cytotoxicity
Funding
- National Natural Science Foundation of China [21371059, 21671068]
- Guangdong Natural Science Foundation [S2012040006270, 2015A030310202]
- Science and Technology Innovation Foundation of Guangdong University Students (Special Foundation for Climbing Plan)
- Engineering Technology Development Center of Advanced Materials & Energy Saving and Emission Reduction in Guangdong Colleges and Universities [2016GCZX007]
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Two new water-soluble metal carboxyl porphyrins, manganese (III) meso-tetrakis (carboxyl) porphyrin and iron (III) meso-tetrakis (carboxyl) porphyrin, were synthesized and characterized. Their interactions with ct-DNA were investigated by UV-Vis titration, fluorescence spectra, viscosity measurement and CD spectra. The results showed they can strongly bind to ct-DNA via outside binding mode. Electrophoresis experiments revealed that both complexes can cleave pBR322 DNA efficiently in the presence of hydrogen peroxide, albeit 2-Mn exhibited a little higher efficiency. The inhibitor tests suggest the oxidative DNA cleavage by these two complexes may involve hydroxyl radical active intermediates. Notably, 2-Mn exhibited considerable photocytotoxicity against Hep G2 cell via triggering a significant generation of ROS and causing disruption of MMP after irradiation.
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