Journal
MOLECULES
Volume 22, Issue 9, Pages -Publisher
MDPI AG
DOI: 10.3390/molecules22091459
Keywords
total triterpenes; Alismatis rhizoma; diuretic activity; component compatibility
Funding
- National Natural Science Foundation of China [U1205022, 81773956, 81503204, 81703692]
- Provincial Health Department Foundation of Fujian [2016-2-29]
- Program for New Century Excellent Talents and Outstanding Young scientific researcher in Fujian Province University from Fujian Education Department
- Subject of Fujian Province Education Hall [JA15242]
- Fujian Province College Students' Innovative Entrepreneurial Training Plan Project [201610393059]
- School Supervision Subject of Fujian University of Traditional Chinese Medicine [X2015011, X2014107]
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Alismatis rhizoma (AR), the dried rhizoma of Alisma orientale Juzepzuk (Alismataceae), is a traditional Chinese medicine. AR is an important part of many prescriptions and is commonly used as a diuretic agent in Asia. This study aimed to evaluate the diuretic effects of total triterpene extract (TTE) and triterpene component compatibility (TCC, the mixture of alisol B 23-acetate, alisol B, alisol A 24-acetate, alisol A, and alisol C 23-acetate) of AR in saline-loaded rats. The optimal diuretic TCC of AR was optimized using a uniform design. Different doses (5, 20, and 40 mg/kg) of TTE and TCC groups (N1-N8) were orally administered to rats. Urinary excretion rate, pH, and electrolyte excretion were measured in the urine of saline-loaded rats. Results showed that TTE doses increased urine volume and electrolyte excretion compared with the control group. All uniformly designed groups of TCC also increased urine excretion. In addition, optimal diuretic TCC was calculated (alisol B 23-acetate: alisol B: alisol A 24-acetate: alisol A: alisol C 23-acetate 7.2:0.6:2.8:3.0:6.4) and further validated by saline-loaded rats. This study demonstrated that TTE presented a notable diuretic effect by increasing Na+, K+, and Cl displacements. The most suitable TTC compatible proportion of alisol B 23-acetate: alisol B: alisol A 24-acetate: alisol A: alisol C 23-acetate for diuretic activity was validated, and triterpenes were the material basis for the diuretic activity of AR.
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