Journal
MOLECULAR SYSTEMS BIOLOGY
Volume 13, Issue 10, Pages -Publisher
WILEY
DOI: 10.15252/msb.20177834
Keywords
CRISPR/Cas; genetic screening; massively parallel lineage tracing; unique molecular identifiers
Categories
Funding
- Academy of Finland [250345]
- Knut and Alice Wallenberg Foundation [KAW 2013.0088]
- Center for Innovative Medicine (CIMED) project grant Growth Control and Cancer
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Loss-of-function screening by CRISPR/Cas9 gene knockout with pooled, lentiviral guide libraries is a widely applicable method for systematic identification of genes contributing to diverse cellular phenotypes. Here, Random Sequence Labels (RSLs) are incorporated into the guide library, which act as unique molecular identifiers (UMIs) to allow massively parallel lineage tracing and lineage dropout screening. RSLs greatly improve the reproducibility of results by increasing both the precision and the accuracy of screens. They reduce the number of cells needed to reach a set statistical power, or allow a more robust screen using the same number of cells.
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