Journal
MOLECULAR NEUROBIOLOGY
Volume 55, Issue 3, Pages 2362-2383Publisher
SPRINGER
DOI: 10.1007/s12035-017-0480-z
Keywords
Benzophenone-3; BP-3; Estrogen receptors; Peroxisome proliferator-activated receptor gamma; Primary neuronal cell cultures
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Funding
- National Science Centre, Poland [2014/13/N/NZ4/04845]
- Institute of Pharmacology Polish Academy of Sciences, Krakow, Poland
- KNOW - Ministry of Science and Higher Education, Poland
- Jagiellonian University
- European Union
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Although benzophenone-3 (BP-3) has frequently been reported to play a role in endocrine disruption, there is insufficient data regarding the impact of BP-3 on the nervous system, including its possible adverse effects on the developing brain. Our study demonstrated that BP-3 caused neurotoxicity and activated apoptosis via an intrinsic pathway involving the loss of mitochondrial membrane potential and the activation of caspases-9 and -3 and kinases p38/MAPK and Gsk3 beta. These biochemical alterations were accompanied by ROS production, increased apoptotic body formation and impaired cell survival, and by an upregulation of the genes involved in apoptosis. The BP-3-induced effects were tissue-specific and age-dependent with the most pronounced effects observed in neocortical cells at 7 days in vitro. BP-3 changed the messenger RNA (mRNA) expression levels of Er alpha, Er beta, Gpr30, and Ppar gamma in a time-dependent manner. At 3 h of exposure, BP-3 downregulated estrogen receptor mRNAs but upregulated Ppar gamma mRNA. After prolonged exposures, BP-3 downregulated the receptor mRNAs except for Er beta mRNA that was upregulated. The BP-3-induced patterns of mRNA expression measured at 6 and 24 h of exposure reflected alterations in the protein levels of the receptors and paralleled their immunofluorescent labeling. Er alpha and Ppar gamma agonists diminished, but Er beta and Gpr30 agonists stimulated the BP-3-induced apoptotic and neurotoxic effects. Receptor antagonists caused the opposite effects, except for ICI 182,780. This is in line with a substantial reduction in the effects of BP-3 in cells with siRNA-silenced Er beta/Gpr30 and the maintenance of BP-3 effects in Er alpha- and Ppar gamma siRNA-transfected cells. We showed for the first time that BP-3-affected mRNA and protein expression levels of Er alpha, Er beta, Gpr30, and Ppar gamma, paralleled BP-3-induced apoptosis and neurotoxicity. Therefore, we suggest that BP-3-evoked apoptosis of neuronal cells is mediated via attenuation of Er alpha/Ppar gamma and stimulation of Er beta/Gpr30 signaling.
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