Journal
BIOMEDICAL MATERIALS
Volume 14, Issue 2, Pages -Publisher
IOP PUBLISHING LTD
DOI: 10.1088/1748-605X/aaf8ed
Keywords
3D printing; hydrogel; chitosan; inflammation; articular cartilage
Funding
- National Key Research and Development Program of China [2018YFB1105600]
- National Natural Science Foundation of China [81572156, 81772326]
- Key Developing Disciplines Program of Shanghai Health System [2015ZB04904]
- China Scholarship Council
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Biological regeneration of articular cartilage continues to be a challenge at present. Functional engineered implants with patient-specific sizes are difficult to achieve. The aim of this study is to fabricate a biocompatible cell-laden hydrogel with a designable structure. Covalent hydrogels were prepared with water soluble hydroxybutyl chitosan (HBC) and oxidized chondroitin sulfate (OCS) via a Schiff-base reaction. With the aid of three-dimensional (3D) bioprinted sacrificial molds, HBC/OCS hydrogel with various structures were obtained. After the material constituent optimization process, an injectable hydrogel with a uniform porous structure of 100 mu m average pore size was developed to form macroporous hydrogel. In vitro and in vivo biocompatibility of optimized HBC/OCS hydrogel were also carefully assessed. The results indicated that human adipose-derived mesenchymal stem cells could be 3D cultured in HBC/OCS hydrogel maintaining good viability. Moreover, the hydrogels were found to trigger the least amount of pro-inflammatory gene expression of macrophage and to inhibit acute immune responses in 7 d. These results demonstrate the potential of HBC/OCS hydrogels as a cell delivery system for cartilage tissue engineering.
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