Journal
MOLECULAR MEDICINE REPORTS
Volume 16, Issue 4, Pages 4045-4053Publisher
SPANDIDOS PUBL LTD
DOI: 10.3892/mmr.2017.7104
Keywords
inflammation; Yunnan Baiyao; paw edema; cyclooxygenase; 5-lipooxygenase; traditional Chinese medicine
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Funding
- National Natural Science Foundation of China [81060086]
- Nature Science Foundation of Yunnan Province [2012FB008]
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The traditional Chinese medicine Yunnan Baiyao (YNB) has been reported to possess antiinflamma-tory properties, however its mechanism of action remains unclear. It was previously reported that YNB ameliorated depression of arachidonic acid (AA) levels in a rat model of collagen-induced arthritis. In the current study, the capacity of YNB to ameliorate inflammation was compared in carrageenan-induced and AA-induced acute inflammation of the rat paw with celecoxib and mizolastine, respectively (n= 24 per group). The capacity of YNB to affect the phospholipase A2 (PLA2)/AA pathway (using reverse transcription-quantitative polymerase chain reaction) and release of inflammatory lipid mediators (by ELISA) were investigated. Celecoxib ameliorated carrageenan-induced paw edema, and mizolastine ameliorated AA-induced rat paw edema. YNB alleviated paw edema and inhibited inflammatory cell infiltration in the two models. YNB inhibited production of 5-LOX AA metabolite leukotriene B4 (LTB4), and suppressed expression of 5-LOX, cytosolic PLA2 (cPLA2), 5-LOX-activating protein, and LTB4 receptor mRNA in the AA-induced inflammation model (P< 0.05). YNB Inhibited the production of the COX-2 AA metabolite prostaglandin E2 (PGE2) and suppressed expression of COX-2, cPLA2, PGE2 mRNA in the carrageenan-induced inflammation mode (P< 0.05). Taken together, the data suggest that modulation of COX and LOX pathways in AA metabolism represent a novel anti-inflammatory mechanism of YNB.
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