Journal
MOLECULAR MEDICINE REPORTS
Volume 16, Issue 3, Pages 2604-2610Publisher
SPANDIDOS PUBL LTD
DOI: 10.3892/mmr.2017.6925
Keywords
pancreatic beta cells; miR-433; glucose; cyclooxygenase 2; cell proliferation
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Pancreatic beta cell dysfunction is a key characteristic in the pathogenesis of diabetes mellitus (DM). MicroRNAs (miRNAs) have been identified to serve a role in DM pathogenesis, but how specific miRNAs regulate glucose-stimulated beta cell functions remain unclear. The present study aimed to explore the effects of miR-433 on cell growth under high-glucose culture conditions and to determine the possible mechanisms involved. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analysis was performed to detect the expression levels of miRNAs in Min-6 pancreatic beta cells cultured in high-glucose medium, which revealed that miR-433 was significantly downregulated. Results from in vitro Cell Counting Kit-8, colony formation and flow cytometry analyses indicated that overexpression of miR-433 may enhance cell viability and proliferation by promoting cell cycle progression and suppressing apoptosis. Furthermore, bioinformatics prediction and luciferase analysis demonstrated that miR-433 was able to inhibit the expression of cyclooxygenase 2 (COX2) through targeting its 3' -UTR. Moreover, knockdown of COX2 expression alleviated the inhibition of cell growth induced by high glucose, similar to overexpression of miR-433. In conclusion, the present results suggested that miR-433 may protect pancreatic beta cells cultured in high glucose, which suggests that miR-433 may have beneficial effects in preventing and treating DM.
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