4.5 Article

Low shear stress-induced autophagy alleviates cell apoptosis in HUVECs

Journal

MOLECULAR MEDICINE REPORTS
Volume 15, Issue 5, Pages 3076-3082

Publisher

SPANDIDOS PUBL LTD
DOI: 10.3892/mmr.2017.6401

Keywords

low shear stress; atherosclerosis; autophagy; apoptosis; mechanism

Funding

  1. Natural Science Foundation of Heilongjiang Province Project [H201345]
  2. Postdoctoral Foundation of Heilongjiang Province Project [LBH-Z11062]

Ask authors/readers for more resources

Low shear stress (LSS) is a well-established risk factor resulting in endothelial apoptosis and atherosclerosis. Autophagy has been reported to be involved in the development of atherosclerosis. However, whether autophagy participates in LSS-induced atherosclerosis remains unclear. The effect of autophagy and its association with apoptosis, in the development of atherosclerosis, remains controversial. Therefore, in the present study, the level and role of autophagy in human umbilical vein endothelial cells (HUVECs) exposed to LSS was examined. The results revealed that LSS increased the formation of autophagosomes and MAP1 light chain 3-like protein (LC3) puncta (as demonstrated by transmission electron microscopy and immunofluorescence), and the protein levels of Beclin-1 and LC3II decreased the expression of p62 [as revealed by western blot analysis (WB)]. Furthermore, the level of p62 decreased when autophagy was induced by rapamycin, and increased when autophagy was inhibited by chloroquine (CQ), which indicated that LSS may serve an important role in inducing autophagy flux. In addition, it was observed that HUVECs treated with LSS underwent apoptotic death, by monitoring the rate of apoptosis and the expression of apoptosis regulator BAX (Bax) and apoptosis regulator Bcl-2 (Bcl-2) (by flow cytometry and WB) and the LSS-induced apoptosis in HUVECs, that was significantly alleviated by pretreatment with rapamycin, partially via a decrease in the level of Bax and an increase in the level of Bcl-2. Pretreatment of HUVECs with CQ markedly increased LSS-induced apoptosis, which was associated with an increased expression of Bax and a decreased expression of Bcl-2. In conclusion, the results of the present study indicate that LSS increases the level of autophagy, which may be through a Bcl-2/Beclin-1-dependent mechanism, which serves a protective role against LSS-induced apoptosis.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.5
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available