Journal
MOLECULAR IMMUNOLOGY
Volume 81, Issue -, Pages 127-134Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.molimm.2016.12.006
Keywords
SLE; cDCs; TREM-1; miRNA-150
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Funding
- Natural Science Foundation of Zhejiang Province [LQ16C080002]
- Research Development Foundation of Wenzhou Medical University [QTJ14025]
- Science and Technology Plan Project of Wenzhou [Y20160179, Y20160191]
- Task of the Department of Education in Zhejiang Province [Y201223758]
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Over activation of conventional dendritic cells (cDCs) contributes to the development of systemic lupus erythematosus (SLE). Triggering receptor expressed on myeloid cells 1 (TREM-1) is emerging as a potent amplifier of the inflammatory responses. We sought to determine the expression level of TREM-1 on cDCs in a mice model of SLE and to identify miRNA which could modulate TREM-1 expression. In the present study, TREM-1 expression in splenocytes and on cDCs was strongly up-regulated in vivo, and was enhanced with LPS stimulation in vitro. Blockade of TREM-1 signal impaired the TLR4-induced cytokines production. These indicated that TREM-1 potently amplified the function of TLR4 which enhanced the inflammation responses. A common set of dysregulated miRNAs (miRNA-98,-150 and-494) were identified in splenocytes of mice. Moreover, the results of bioinformatics and the immunoblotting, demonstrated that miRNA-150 inhibited the expression of TREM-1. Together, these data suggested that TREM-1 signaling pathway may be a therapeutic target to prevent the effects of the inflammatory cDCs in SLE and miRNA-150 serves as the important regulator. (C) 2016 Elsevier Ltd. All rights reserved.
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