Journal
JACC-BASIC TO TRANSLATIONAL SCIENCE
Volume 4, Issue 1, Pages 98-112Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.jacbts.2018.10.005
Keywords
aldehyde dehydrogenase; angiogenesis; ischemia; progenitor cells; type 2 diabetes
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Funding
- Canadian Institutes for Health Research [387189, 153293]
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Detection of vascular regenerative cell exhaustion is required to combat ischemic complications during type 2 diabetes mellitus (T2D). We used high aldehyde dehydrogenase (ALDH) activity and surface marker co-expression to develop a high-throughput flow cytometry-based assay to quantify circulating proangiogenic and proinflammatory cell content in the peripheral blood of individuals with T2D. Circulating proangiogenic monocytes expressing anti-inflammatory M2 markers were decreased in patients with T2D. Individuals with longer duration of T2D exhibited reduced frequencies of circulating proangiogenic ALDH(hi)CD34+ progenitor cells with primitive (CD133) and migratory (CXCR4) phenotypes. This approach consistently detected increased inflammatory cell burden and decreased provascular progenitor content in individuals with T2D. (C) 2019 The Authors. Published by Elsevier on behalf of the American College of Cardiology Foundation.
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