Journal
MOLECULAR CRYSTALS AND LIQUID CRYSTALS
Volume 648, Issue 1, Pages 236-243Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/15421406.2017.1302580
Keywords
Biopolymers; biodegradable polymers; mechanical properties; nanoindentation; PHA; PHB
Funding
- Russian Science Foundation [15-15-10014]
- Russian Science Foundation [15-15-10014] Funding Source: Russian Science Foundation
Ask authors/readers for more resources
A study of in vitro biodegradation of poly(3-hydroxybutyrate) (PHB) in model conditions was performed. The porcine pancreatic lipase solutions at different concentrations in the two buffer systems (phosphate-buffered saline (PBS) and simulating body fluid (SBF)) were chosen as model biodegradation media. At first, optimal concentration of pancreatic lipase (0.25 mg/ml in PBS) was determined: in these media the decomposition of PHB films realized faster according to the data of gravimetric analysis. Differential scanning calorimetry showed an increase in the crystallinity of the samples (from 49% to 59%) after enzymatic degradation. These data are confirmed by method of nanoindentation, where the increase of the Young's modulus during the degradation (from 1.37 GPa to 4.4 GPa) was shown. This is due to the crystallization of the amorphous polymer component, and its decomposition and dissolving. During biodegradation three types of polymer ultrastructure changes were observed on the surfaces of the films: appearance of new lamellae, disappearance of lamellae and disintegration of lamellae into shorter fragments.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available