Journal
MOLECULAR CELL
Volume 67, Issue 2, Pages 282-+Publisher
CELL PRESS
DOI: 10.1016/j.molcel.2017.06.018
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Funding
- MEXT [23115005]
- JSPS [16H04746]
- JST CREST grant [JPMJCR15G2]
- Grants-in-Aid for Scientific Research [16J07205, 16K15095, 16H04746] Funding Source: KAKEN
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The eukaryotic genome is organized within cells as chromatin. For proper information output, higher-order chromatin structures can be regulated dynamically. How such structures form and behave in various cellular processes remains unclear. Here, by combining super-resolution imaging (photoactivated localization microscopy [PALM]) and single-nucleosome tracking, we developed a nuclear imaging system to visualize the higher-order structures along with their dynamics in live mammalian cells. We demonstrated that nucleosomes form compact domains with a peak diameter of similar to 160 nm and move coherently in live cells. The heterochromatin-rich regions showed more domains and less movement. With cell differentiation, the domains became more apparent, with reduced dynamics. Furthermore, various perturbation experiments indicated that they are organized by a combination of factors, including cohesin and nucleosome-nucleosome interactions. Notably, we observed the domains during mitosis, suggesting that they act as building blocks of chromosomes and may serve as information units throughout the cell cycle.
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