Journal
MOLECULAR CELL
Volume 66, Issue 3, Pages 420-+Publisher
CELL PRESS
DOI: 10.1016/j.molcel.2017.04.010
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Funding
- Villum Foundation
- Danish Independent Research Council \ Natural Sciences [12-125524]
- Danish Diabetes Academy - Novo Nordisk Foundation
- Lundbeck Foundation [R118-A11639]
- Augustinus Foundation
- Novo Nordisk Foundation [NNF15OC0014136]
- Medical Research Council, UK [MR/L007150/1]
- Biotechnology and Biological Research Council, UK [BB/J004480/1]
- Biotechnology and Biological Sciences Research Council [BBS/E/B/000C0404, BBS/E/B/000C0405] Funding Source: researchfish
- Medical Research Council [MR/L007150/1] Funding Source: researchfish
- Novo Nordisk Fonden [NNF15OC0014136, NNF15OC0018212] Funding Source: researchfish
- Villum Fonden [00007292] Funding Source: researchfish
- BBSRC [BBS/E/B/000C0405, BBS/E/B/000C0404] Funding Source: UKRI
- MRC [MR/L007150/1] Funding Source: UKRI
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Interactions between transcriptional promoters and their distal regulatory elements play an important role in transcriptional regulation; however, the extent to which these interactions are subject to rapid modulations in response to signals is unknown. Here, we use promoter capture Hi-C to demonstrate a rapid reorganization of promoter-anchored chromatin loops within 4 hr after inducing differentiation of 3T3-L1 preadipocytes. The establishment of new promoter-enhancer loops is tightly coupled to activation of poised (histone H3 lysine 4 mono-and dimethylated) enhancers, as evidenced by the acquisition of histone H3 lysine 27 acetylation and the binding of MED1, SMC1, and P300 proteins to these regions, as well as to activation of target genes. Intriguingly, formation of loops connecting activated enhancers and promoters is also associated with extensive recruitment of corepressors such as NCoR and HDACs, indicating that this class of coregulators may play a previously unrecognized role during enhancer activation.
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