Journal
MOLECULAR CELL
Volume 68, Issue 1, Pages 44-59Publisher
CELL PRESS
DOI: 10.1016/j.molcel.2017.09.017
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Funding
- National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK) Award [F30-DK103359]
- National Institutes of Health through National Human Genome Research Institute [R00-HG008171]
- Kimmel Foundation
- Melanoma Research Alliance
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The clustered regularly interspaced short palindromic repeats (CRISPR)-Cas nuclease system is a powerful tool for genome editing, and its simple programmability has enabled high-throughput genetic and epigenetic studies. These high-throughput approaches offer investigators a toolkit for functional interrogation of not only protein-coding genes but also noncoding DNA. Historically, noncoding DNA has lacked the detailed characterization that has been applied to protein-coding genes in large part because there has not been a robust set of methodologies for perturbing these regions. Although the majority of high-throughput CRISPR screens have focused on the coding genome to date, an increasing number of CRISPR screens targeting noncoding genomic regions continue to emerge. Here, we review high-throughput CRISPR-based approaches to uncover and understand functional elements within the noncoding genome and discuss practical aspects of noncoding library design and screen analysis.
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