Journal
MOLECULAR CELL
Volume 66, Issue 5, Pages 721-+Publisher
CELL PRESS
DOI: 10.1016/j.molcel.2017.04.025
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Funding
- Momentum Fund (Ramot at Tel Aviv University)
- European Research Council StG program [336079]
- Israel Science Foundation [268/14]
- Israeli Ministry of Health [9988-3]
- European Research Council (ERC) [336079] Funding Source: European Research Council (ERC)
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A major limitation in using bacteriophage-based applications is their narrow host range. Approaches for extending the host range have focused primarily on lytic phages in hosts supporting their propagation rather than approaches for extending the ability of DNA transduction into phage-restrictive hosts. To extend the host range of T7 phage for DNA transduction, we have designed hybrid particles displaying various phage tail/tail fiber proteins. These modular particles were programmed to package and transduce DNA into hosts that restrict T7 phage propagation. We have also developed an innovative generalizable platform that considerably enhances DNA transfer into new hosts by artificially selecting tails that efficiently transduce DNA. In addition, we have demonstrated that the hybrid particles transduce desired DNA into desired hosts. This study thus critically extends and improves the ability of the particles to transduce DNA into novel phage-restrictive hosts, providing a platform for myriad applications that require this ability.
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