Journal
MOLECULAR CELL
Volume 68, Issue 2, Pages 446-+Publisher
CELL PRESS
DOI: 10.1016/j.molcel.2017.09.018
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Funding
- NIH [R35GM118015, R01GM107239]
- Swedish Cancer Society
- Swedish Research Council
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The checkpoint kinase Rad53 is activated during replication stress to prevent fork collapse, an essential but poorly understood process. Here we show that Rad53 couples leading-and lagging-strand synthesis under replication stress. In rad53-1 cells stressed by dNTP depletion, the replicative DNA helicase, MCM, and the leading-strand DNA polymerase, Pol epsilon, move beyond the site of DNA synthesis, likely unwinding template DNA. Remarkably, DNA synthesis progresses further along the lagging strand than the leading strand, resulting in the exposure of long stretches of single-stranded leading-strand template. The asymmetric DNA synthesis in rad53-1 cells is suppressed by elevated levels of dNTPs in vivo, and the activity of Pol epsilon is compromised more than lagging-strand polymerase Pol delta at lowdNTP concentrations in vitro. Therefore, we propose that Rad53 prevents the generation of excessive ssDNA under replication stress by coordinating DNA unwinding with synthesis of both strands.
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