Journal
MOLECULAR BREEDING
Volume 37, Issue 3, Pages -Publisher
SPRINGER
DOI: 10.1007/s11032-017-0633-9
Keywords
C-class MADS-box gene; Double-flower; GsAG1; Japanese gentian; Molecular marker
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Funding
- Science and Technology Research Promotion Program for Agriculture, Forestry, Fisheries and Food Industry [27030C]
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Double flowers are valuable floral traits in most floricultural plants. We recently revealed that a double-flowered mutant of Gentiana scabra was caused by an insertion of a retrotransposable element (Tgs1) into GsAG1, one of the C-class MADS-box genes in gentian. In this study, we developed a PCR-based molecular DNA marker to distinguish double-and single-flower phenotypes at the young seedling stage in Japanese gentian plants. To test the validity of the markers, 17 F-2 populations were produced by selfing F-1 plants crossed between the double-flower mutant and seven breeding lines. Multiplex PCR demonstrated that the Tgs1 insertion in GsAG1 cosegregated with the double-flower phenotype in two F-2 populations, indicating that the PCR-based DNA marker was useful to discriminate between double-and single-flower phenotypes in advance of flowering in Japanese gentian. Given that Japanese gentians lack variation in flower shape and require a long breeding period, the DNA marker developed here will be helpful for efficient breeding of double-flowered cultivars in the future.
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