Journal
MOLECULAR BREEDING
Volume 37, Issue 6, Pages -Publisher
SPRINGER
DOI: 10.1007/s11032-017-0670-4
Keywords
Powdery mildew; Marker-assisted transfer; Wheat; Pm4e
Categories
Funding
- Natural Science Foundation of China [31301308, 30025030, 31430064]
- Special Transgenic Fund [2014ZX0800906B]
- JSNSF [BK20130679]
- Jiangsu Collaborative Innovation Initiative for Modern Crop Production
- '111' project [B08025]
Ask authors/readers for more resources
Powdery mildew, a wheat (Triticum aestivum L.) foliar disease caused by Blumeria graminis (DC.) E.O. Speer f. sp. tritici, imposes a constant challenge on wheat production in areas with cool or maritime climates. This study was conducted to identify and transfer the resistance gene in the newly identified common wheat accession 'D29'. Genetic analysis of the F-2 population derived from a cross of D29 with the susceptible elite cultivar Y158 suggested a single dominant gene is responsible for the powdery mildew resistance in this germplasm. This gene was mapped to chromosome 2AL in a region flanked by microsatellite markers Xgdm93 and Xhbg327, and co-segregated with sequence-tagged site (STS) markers Xsts_bcd1231 and TaAetPR5. An allelic test indicated that the D29 gene was allelic to the Pm4 locus. To further evaluate the resistance conferred by this gene and develop new germplasms for breeding, this gene, as well as Pm4a and Pm4b, was transferred to Y158 through backcross and marker-assisted selection. In the resistance spectrum analysis, the D29 gene displayed a resistance spectrum distinguishable from the other Pm4 alleles, including Pm4a, Pm4b, and Pm4c, and thus was designated as Pm4e. The identification of new allelic variation at the Pm4 locus is important for understanding the resistance gene evolution and for breeding wheat cultivars with powdery mildew resistance.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available