Journal
MOLECULAR BIOSYSTEMS
Volume 13, Issue 10, Pages 1940-1945Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c7mb00245a
Keywords
-
Categories
Funding
- Dutch Kidney Foundation [CP 10.12]
- Dutch Kidney Foundation
- Ministry of Economy and Competitiveness (Spain) [CTQ2013-48740-P]
- University of Alcala
- Leiden University Medical Center
Ask authors/readers for more resources
Metabolic profiling provides an unbiased view of the physiological status of an organism as a function'' of the metabolic composition of a measured sample. Here, we propose a simple LC-MS based workflow for metabolic profiling of volume-restricted samples, namely individual 20 mu m-thick histological sections of a mouse kidney. The main idea of this workflow is to re-use the material after an RPLC-MS run, namely using the volume remaining in the vial after injection, and then introducing a phase changing step to enable HILIC-MS analysis. To test the applicability of the workflow and its ability to extract valuable biological information, we applied it to an animal model of polycystic kidney disease (PKD).
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available