Journal
MOLECULAR BIOLOGY OF THE CELL
Volume 28, Issue 4, Pages 545-553Publisher
AMER SOC CELL BIOLOGY
DOI: 10.1091/mbc.E16-09-0628
Keywords
-
Categories
Funding
- National Natural Science Foundation of China [31171007, 31371078]
- Priority Academic Program Development of Jiangsu Provincial Department of Education, Scientific Innovation Project [KYZZ_0354]
Ask authors/readers for more resources
Microtubule (MT) organization is essential for many cellular events, including mitosis, migration, and cell polarity. Fidgetin (Fign), an ATP-dependent, MT-severing protein, contributes to the regulation of MT configuration by cutting and trimming MT polymers. Functions of Fign have been indicated in neurite outgrowth, mitosis, meiosis, and cellular migration. Here we focus on migration of astrocytes. We find that Fign plays an essential role in cultured astrocyte migration by preferentially targeting MTs (or regions of MTs) that are rich in tyrosinated tubulin, a marker for especially dynamic MTs or especially dynamic regions of MTs. Inhibition of cellular migration induced by Fign knockdown can be rescued with concomitant knockdown of kinesin-12, a motor protein best known for its role in mitosis. We propose a novel working model for MT reconfiguration underlying cellular migration elicited by the functional cooperation of two distinct MT-related proteins.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available