Journal
MOLECULAR AND CELLULAR PROBES
Volume 33, Issue -, Pages 4-7Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.mcp.2017.02.001
Keywords
Acute hepatopancreatic necrosis disease; Vibrio owensii; Recombinase polymerase amplification; RPA; Diagnosis
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Funding
- National Natural Science Foundation of China [41376135, 31570112, 31601570]
- Doctoral Fund of Ministry of Education of China [20133104110006]
- Innovation Program of Shanghai Municipal Education Commission, China [14ZZ144]
- Funding Program for Outstanding Dissertations of Shanghai Ocean University
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A rapid and sensitive AHPND-RPA assay was developed for the specific detection of the AHPND-causing Vibrio owensii. The AHPND-RPA detected as few as 2 copies per reaction in 9.02 +/- 0.66 min at 39 degrees C, and showed 100% positive predictive value and negative predictive value for AHPND diagnosis. (C) 2017 Elsevier Ltd. All rights reserved.
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