Journal
MOLECULAR AND CELLULAR BIOCHEMISTRY
Volume 428, Issue 1-2, Pages 119-128Publisher
SPRINGER
DOI: 10.1007/s11010-016-2922-8
Keywords
Recombinant IL-18; ICAM-1; VCAM-1; PPAR-gamma; PDTC
Categories
Funding
- Department of Biotechnology, New Delhi, India
Ask authors/readers for more resources
Focal recruitment of monocytes and lymphocytes is one of the earliest detectable cellular responses in atherosclerotic lesion formation. Endothelium may regulate leukocyte recruitment by expressing specific adhesion molecules. Interleukin-18 is a proinflammatory cytokine that plays an important role in vascular pathologies. The present study highlights the modulation of adhesion molecules and PPAR-gamma by IL-18 and proposes a novel feedback mechanism by which PPAR-gamma may regulate IL-18 expression. Three groups of normal chow diet-fed, male Apo E-/- mice, aged 12 weeks (n = 6/group) were employed: Gp I, phosphate-buffered saline (PBS) (2 mo): Gp II, recombinant IL-18 (rIL-18) (1 mo) followed by PBS (1 mo); Gp III, rIL-18 (1 mo) followed by pyrrolidine dithiocarbamate (PDTC) (1 mo). Significantly augmented mRNA expression of ICAM-1 (similar to 5.7-fold), VCAM-1 (similar to 3.6-fold), and NF-kappa B (similar to 7-fold) was observed in Gp II mice as compared to Gp I, whereas PPAR-gamma expression was not altered. PDTC treatment caused a significant downregulation of ICAM-1 (similar to 4.2-fold), VCAM-1(similar to 2-fold), and NF-kappa B (similar to 4.5-fold) and upregulation of PPAR-gamma expression (similar to 5-fold) in Gp III mice. A similar trend was observed in protein expression. In vivo imaging results demonstrated a marked increase in probe (CF750 dye conjugated to VCAM-1 antibody) fluorescence intensity for VCAM-1 expression in Gp II mice, whereas it was moderately decreased in Gp III. PPAR-gamma was found to significantly downregulate both IL-18 levels and IL-18-induced adhesion molecules. The underlying mechanism was found to be via inhibition of NF-kappa B activity by PDTC, thereby leading to decreased adherence of monocytes to the activated endothelial cells and a step to halt the progression and development of atherosclerotic lesions.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available